Veterinary Biomedical and Clinical Journal

Treatmant of Malunion Comminuted Fracture Os Tibia in Maltese Dog

2023-03-17T10:55:40+00:00

A comminuted fracture is a type of fracture where the affected bone is divided into three or more pieces of different sizes. Improper handling of fractures can potentially lead to malunion and chronic pain. In malunion, the fractured bones are fused but in an abnormal position. Malunion occurs due to inaccurate bone reduction or excessive mobilization during the healing period. The purpose of this article is to describe a surgical method in handling a case of comminuted fracture of the tibia in a dog. A Maltese dog, 3 kg weight, was brought to the clinic due to gait immobility with lifting the right hind leg and limping. In the previous month, the dog suffered from a comminuted fracture of the right tibia and had been treated with plates and screws. During the physical examination, the dog showed good general condition, while the rear right leg was raised. Radiographic results at the medio-lateral and caudo-cranial projection showed the malunion bone due to a shift in the fracture fragment of the diaphysis of right tibia after plate and screw implantation in the comminuted fracture. In the present correction, the plate and screw were replaced by intra-medullary pins combined with wire implementing the Open Reduction and Internal Fixation (ORIF) method. Atropine sulfate premedication was given subcutaneously at 0.02 mg/kgBW. Anesthesia was induced using a combination of 10% ketamine and 2% xylazine, and maintained by a 2% isoflurane inhalation. Postoperative therapy given included Penicillin Procaine G®, Dexamethasone® (0.5 mg/kgBW,IM), Tramadol® (5 mg/kgBW,IM), Viccillin® (10 mg/kgBW,IM), and Cyanocobalamin® (100 mcg/ kgBW, IM). There was no postoperative complication observable, and the patient was brought home on the third day post-surgery when its affected feet was able to touch the floor. The study showed that ORIF method could be performed to correct malunion in a case of comminuted tibia fracture.

The Identification of Hysterothlacium on Sixbar Groupers (Epinephelus sexfasciatus) in The Territorial Waters of East Java Using Sequencing Method

2023-05-12T04:10:12+00:00

This study aimed to identify the parasitic nematode Hysterothylacium sp. using the sequencing method. A sampling of Hysterothylacium sp. was taken from 60 striped grouper fish (Ephinephelus sexfasciatus) and has characteristics such as brown color, booring teeth on the anterior, a simple digestive system including the esophagus, stomach (ventriculus), small intestine and does not have mucron on the posterior. Specific primers for the COX-2 mtDNA gene (210F and 211R) were used for DNA amplification (Polymerase Chain Reaction) and DNA sequencing with a length of 629bp. The positive result of the PCR method (sample S) was sequenced. Then, the results of the sequencing method were compared with the reference DNA sequences KY065298.1, MF120253.1, MF120248.1, and KU886687.1. Furthermore, Homology and kinship tests were carried out using GenBank (NCBI). Results showed a 97% similarity of the sample with Hysterothylacium amoyense cytochrome oxidase subunit II (COX-2) gene MF120253.1.

The Potentials of Robusta Coffee Seed Extract as Antioxidant on Kidney Histopathology in Mice Exposed to Monosodium Glutamate

2023-06-20T05:51:36+00:00

The purpose of this study was to determine the potential of administration robusta coffee extract as an antioxidant on the renal histopathology in mice exposed to monosodium glutamate (MSG) orally. This study used 20 adult male rats with Balb C strain divided into 5 experimental groups, namely K + (MSG 0.12 mg / head and Vitamin C 6 mg/mice), K- (MSG 0.12 mg/mice and Sodium carboxymethyl cellulose (CMC Na) 0,1 ml/mice), P1 (MSG 0.12 mg/mice and Robusta coffee extract 0.1 mg/mice), P2 (MSG 0.12 mg/mice and Robusta coffee extract 0.2 mg/mice), and P3 (MSG 0.12 mg/mice and 0.4 mg Robusta coffee extract mg/mice). The experimental group was treated every day at intervals between MSG and Vitamin C, CMC Na, and Robusta coffee extract for 60 min, the treatment was carried out for 42 days. Kidney histopathology scores were analyzed using the Saphiro-Wilk and Levene test to evaluate homogeneity, followed by the Kruskal Wallis test. The results of the Saphiro Wilk and Levene statistical analysis obtained p<0.05, thus the data continued with the Kruskal Wallis test scores of kidney cell damage obtained 0.043 which showed that the data were significant. The mean score of kidney cell damage in the treatment groups was K+ 1.60; K- 1.63; P1 1.70; P2 1.63; and P3 1.73. The conclusion is that Robusta coffee bean extract did not show a decrease in kidney cell damage that was different between treatment groups. Further research is needed to find out about the effect of different dosages of Robusta Coffee Extract to different organs

Effect of Oregano (Origanum vulgare) Extract on Kidney Histomorphometry and Histopathology in Mice (Mus musculus) Colibacillosis Model

2023-06-20T05:46:25+00:00

Colibacillosis is a disease that infects the digestive tract due to infection with the pathogen Escherichia coli. E. coli is able to cause bacteremia so that E. coli can reach the kidney through blood flowing to the kidney. To reduce the side effects of treatment using antibiotics, the herbal medicine oregano (Origanum vulgare) which contains phenols (carvacrol and thymol) works as an antibacterial. The benefits of this research are as a source of information and the basis for further research related to the use of oregano extract in the treatment of colibacillosis due to E. coli infection. The study used 20 male mice, divided into 5 applicable groups, namely K-, healthy mice were not given any treatment; K+ was induced by Streptomycin 20 mg and infected with E.coli 3 x 10⁹ CFU/ml, 0.5 ml; P1 was induced by Streptomycin 20 mg and infected with E.coli 3 x 10⁹ CFU/ml, 0.5 ml + Origanum vulgare extract 25 mg/kg; P2 was induced by Streptomycin 20 mg and infected with Escherichia coli 3 x 10⁹ CFU/ml, 0.5ml + Origanum vulgare extract 50mg/kg; P3 was induced by Streptomycin 20 mg and infected with E.coli 3 x 10⁹ CFU/ml, 0.5 ml + Origanum vulgare extract 100 mg/kg. Kidney was taken for histopathological preparations with hematoxylin eosin (HE) staining. After that, it was analyzed using Kruskal Wallis and continued with Mann Whitney. Histomorphometry semiquantitative measures the width of the Bowman's glomerular capsule and the length of the tubular lumen and then analyzed using One Way Analysis of Variance (ANOVA). The results of this study showed that oregano extract had an effect on histomorphometry and histopathology of renal, with an optimal dose is 5 mg/mice.

Identification and The Prevalence of Gastrointestinal Endoparasite in Long-Tailed Macaque (Macaca Fascicularis) in Wonorejo and Gunung Anyar Mangrove Eco-Tourism Surabaya

2023-05-27T17:49:58+00:00

This study aimed to determine the types of endoparasites and their prevalence in Macaca fascicularis in the Wonorejo and Gunung Anyar mangrove forests, Surabaya. A total of 90 samples of Macaca fascicularis faeces were collected from two different mangrove forests, 45 samples from Wonorejo and 45 from Gunung Anyar. All samples were examined using sedimentation and floating methods to identify endoparasite species. Further, the number of eggs and oocysts per gram of feces was counted using the McMaster method. Results showed that 26.67% (12/45), and 22.2% (10/45) of the faecal samples were detected positive for endoparasite at Wonorejo and Gunung Anyar mangrove forests, respectively. The prevalence of parasitosis in M. fascicularis from Wonorejo was 17.7% (8/45) for Taenia sp. and 8.8% (4/45) for Balantidium coli cyst. On the other hand, M. fascicularis from Gunung Anyar was infested by Trichuris sp. at a prevalence of 22.2% (10/45). The total number of eggs and cysts ranged from 30 – 400 per gram of faecal samples. The study provides new knowledge on the parasitic burdens among long-tailed macaques in East Java.

Identification and Prevalence of Endoparasites in Timor Deer (Cervustimorensis) at Maliran Deer Captive Breeding Blitar

2023-06-20T08:36:40+00:00

Deer populations in captive breedings have the potential to develop health issues such as protozoa and helminth infections. Poor sanitation and environmental conditions are among the external factors facilitate the transmission of endoparasites within deer population in a captivity. The aim of this study was to identify and determine the prevalence of endoparasite infestation in Timor deer (Cervus timorensis) in Maliran Captive Breeding, Blitar, Indonesia. Samples of 102 fresh feces, were taken during the rainy season from March to May 2021, and sent to Laboratory of Parasitology, Veterinary Medicine Faculty, Universitas Airlangga for fecal examination using sedimentation, flotation, as well as McMaster methods to determine the prevalence and the intensity of infestations. Results showed that 30 samples were detected positive, 7 samples (6.86%) were positive of Haemonchus spp. at the egg stage (range of 30-60 EPG; mild infestation); and 23 samples (22.55%) were positive of Eimeria spp. in oocyst stage (range of 200-800 OPG; mild to moderate infestation). This study provides a reference for the eradication of Haemonchiasis in the deer population in the study area.