Effect of Torbangun Leaf (Coleus ambonicus, L.) Extract Feeding on Cisplatin-Induced in Wistar Rats (Rattus norvegicus)

Gambaran Histopatologi Ginjal yang Diinduksi Cisplatin dan Ekstrak Daun Torbangun (Coleus ambonicus, L.) pada Tikus Wistar (Rattus norvegicus)

Abstract

This study aimed to analyze the effect of torbangun leaf extract (Coleus ambonicus, L.) on kidney histopathology in cisplatin-induced Wistar rats. The design used was Completely Randomized Design (CRD) with three different treatments and six repetitions. The rats used were 18 male Wistar (Rattus norvegicus) rats, bodyweight 150-200 grams and 2-3 months of age. Treatment P0 for Wistar rats given aquadest ad libitum, P1 for Wistar rats injected with Cisplatin on day 4, intraperitoneally at a dose of 7 mg/kg with 1% of CMC NA suspension and P2 for Wistar rats given Torbangun leaf extract orally at a dose of 100 mg/kg BW for seven days. On day 4, groups P1 and P2 were injected with Cisplatin intraperitoneally at a 7 mg/kg BW. The non-parametric data from the study were statistically tested with the Kruskal Wallis test and the post hoc test in the form of the Mann-Whitney test. The test results from statistical analysis showed that the Torbangun leaf extract could reduce tissue damage by significantly reducing hemorrhage, congestion, and vasculitis in the treatment group