Sedative Effect of Lemongrass (Cymbopogon nardus) Aromatherapy through Inhalation Route on Mice (Mus musculus) Behavior

Authors

Hilmy Luqman Surachmat
Veterinary Medicine Study Program, Faculty of Medicine, Padjadjaran University, Jatinangor, West Java 45363, Indonesia
Eko Fuji Arianto
Department of Biomedical Sciences, Faculty of Medicine, Padjadjaran University, Jatinangor, West Java 45363, Indonesia
Shafia Khairani
Department of Biomedical Sciences, Faculty of Medicine, Padjadjaran University, Jatinangor, West Java 45363, Indonesia
Page: 70-78, Abstract views: 27, PDF downloads: 13

DOI:

https://doi.org/10.21776/ub.VetBioClinJ.2024.006.02.3

Keywords:

aromatherapy, Chimney test, Cymbopogon nardus, essential oil, sedative

Abstract

Aromatherapy is an alternative practice in natural medicine that uses essential oil derived from various plants. Essential oil is found in over 17,000 plant species, including lemongrass (Cymbopogon nardus). Lemongrass essential oil contains several active components with potential sedative effects, such as linalool. Therefore, this study aimed to evaluate the potential sedative effect of lemongrass essential oil aromatherapy through inhalation route as an alternative preparation. Mice (Mus musculus) were divided into 5 groups, namely negative and positive control (Diazepam 0.012 mg/kg), as well as treatment 1 (P1, 30-minute exposure), 2 (P2, 60-minute exposure), and 3 (P3, 90-minute exposure). Essential oil of lemongrass was extracted using the distillation method, followed by phytochemical testing. Behavioral observations were carried out using Chimney test method. The results of Chimney test showed that lemongrass aromatherapy had no sedative effect. This result was evident by the insignificant differences (P > 0.05) between negative control groups and both P1 and P3, as well as P1 and P3. Furthermore, P2 showed a potential stimulant effect based on Chimney test results.

References

  • A’yun. Q.. Hermana. B.. & Kalsum. U. (2020). Analisis Rendemen Minyak Atsiri Serai Wangi (Cymbopogon Nardus (L.) Pada Beberapa Varietas. Jurnal Pertanian Presisi (Journal of Precision Agriculture). 4(2). 160–173. Https://Doi.Org/10.35760/Jpp.2020.V4i2.3343
  • Ahmad Kamal. H. Z.. Tuan Ismail. T. N. N.. Arief. E. M.. & Ponnuraj. K. T. (2020). Antimicrobial Activities Of Citronella (Cymbopogon Nardus) Essential Oil Against Several Oral Pathogens And Its Volatile Compounds. Padjadjaran Journal Of Dentistry. 32(1). 1. Https://Doi.Org/10.24198/Pjd.Vol32no1.24966
  • Ali. B.. Al-Wabel. N. A.. Shams. S.. Ahamad. A.. Khan. S. A.. & Anwar. F. (2015). Essential Oils Used In Aromatherapy: A Systemic Review. Asian Pacific Journal Of Tropical Biomedicine. 5(8). 601–611. Https://doi.org/10.1016/J.Apjtb.2015.05.007
  • Bagetta. G.. Cosentino. M.. & Sakurada. T. (2016). Aromatherapy Basic Mechanisms And Evidence-Based Clinical Use.
  • Baldwin. A. L.. & Chea. I. (2018). Effect Of Aromatherapy On Equine Heart Rate Variability. Journal Of Equine Veterinary Science. 68. 46–50. Https://Doi.Org/10.1016/J.Jevs.2018.05.213
  • Buckle. J. (2014). Clinical Aromatherapy: Essential Oils In Healthcare. In Clinical Aromatherapy: Essential Oils In Healthcare. Elsevier Inc. Https://Doi.Org/10.1016/C20120025781
  • Buckle. J. (2015). Clinical Aromatherapy Essential Oils In Healthcare. Elsevier. Https://Doi.Org/10.1016/C20120025781
  • Costa. C. A. R. De A.. Kohn. D. O.. De Lima. V. M.. Gargano. A. C.. Flório. J. C.. & Costa. M. (2011). The Gabaergic System Contributes To The Anxiolytic-Like Effect Of Essential Oil From Cymbopogon Citratus (Lemongrass). Journal Of Ethnopharmacology. 137(1). 828–836. Https://Doi.Org/10.1016/J.Jep.2011.07.003
  • Davis. S. L. C. (2019). CNS Stimulants ­ Overview Of Indications ­ Overdose ­ Clinical Guidelines. 217–229.
  • Ebani. V. V.. & Mancianti. F. (2020). Use Of Essential Oils In Veterinary Medicine To Combat Bacterial And Fungal Infections. Veterinary Sciences. 7(4). 1–35. Https://Doi.Org/10.3390/Vetsci7040193
  • Ferguson. C. E.. Kleinman. H. F.. & Browning. J. (2013). Effect Of Lavender Aromatherapy On Acute-Stressed Horses. Journal Of Equine Veterinary Science. 33(1). 67–69. Https://Doi.Org/10.1016/J.Jevs.2012.04.014
  • Hadinoto. I.. Kuswono. E.. Marlina. A.. Setiawati. A.. Farmasi. F.. & Widya. U. (2012). Uji Efek Sedatif Dari Minyak Clary Sage ( Salvia Sclalarea . L ) Pada Mencit Jantan Secara Olfactory Aromatherapi Sedative Effect Of Clary Sage ( Salvia Sclalarea . L ) On Mice Using An Olfactory Aromatherapy. Fakultas Farmasi Unika Widya Mandala. 46.
  • Katzung. B. G.. Masters. S. B.. & Trevor. A. J. (2012). Basic And Clinical Pharmacology (Katzung & Trevor) 12th Ed.
  • Kaur. H.. Bhardwaj. U.. & Kaur. R. (2021). Cymbopogon Nardus Essential Oil: A Comprehensive Review On Its Chemistry And Bioactivity. Journal Of Essential Oil Research. 33(3). 205–220. Https://Doi.Org/10.1080/10412905.2021.1871976
  • Khoirunnisa. I.. & Sumiwi. S. A. (2019). Review Artikel: Peran Flavonoid Pada Berbagai Aktifitas Farmakologi. Farmaka. 17(2). 131–142. Https://Jurnal.Unpad.Ac.Id/Farmaka/Article/View/21922
  • Kiani. H. S.. Ali. A.. Zahra. S.. Hassan. Z. U.. Kubra. K. T.. Azam. M.. & Zahid. H. F. (2022). Phytochemical Composition And Pharmacological Potential Of Lemongrass (Cymbopogon) And Impact On Gut Microbiota. Appliedchem. 2(4). 229–246. Https://Doi.Org/10.3390/Appliedchem2040016
  • Ludwiczuk. A.. Skalicka-Woźniak. K.. & Georgiev. M. I. (2017). Terpenoids. In Pharmacognosy (Pp. 233–266). Elsevier. Https://Doi.Org/10.1016/B978-0-12-802104-0.00011-1
  • Najmah. N.. Fitria. R.. & Kurniawati. E. (2023). SKRINING FITOKIMIA. TOTAL FLAVONOID DAN FENOLIK DAUN SEREH WANGI (Cymbopogon Nardus (L.) Rendle). Jurnal Crystal : Publikasi Penelitian Kimia Dan Terapannya. 5(1). 62–70. Https://Doi.Org/10.36526/Jc.V5i1.2642
  • Nieoczym. D.. Socała. K.. & Wlaź. P. (2018). Assessment Of The Anticonvulsant Potency Of Ursolic Acid In Seizure Threshold Tests In Mice. Neurochemical Research. 43(5). 995–1002. Https://Doi.Org/10.1007/S11064-018-2505-Z
  • Novitasari. P. R.. Nuraisyah. F.. Prihatmadi. F. A.. Nugroho. A. D.. Yudhana. A.. & Akbar. S. A. (2023). Solvent Effects On Phytochemical Screening Test Of Red Lemongrass (Cymbopogon Nardus (L.) Rendl.) Extract And Its Potential As Antidiabetic Agent. Journal Of Food And Pharmaceutical Sciences. 11(1). 788–794. Https://Doi.Org/10.22146/Jfps.6310
  • Sari. E. K.. Dellima. B. R. E. M.. & Hondro. K. K. (2023). Antibacterial Activity Test Of Ethanol Extract Of Lemongrass Leaves (Cymbopogon Nardus (L.) Rendle) Against Staphylococcus Epidermidis. In Atlantis Press (Pp. 51–58). Https://Doi.Org/10.2991/978-94-6463-190-6_8
  • Solekha. R.. Setiyowati. P. A. I.. Mahaputra. S. B. S.. Kusumanegara. & Sari. C. T. U. (2022). Phytochemical Screening Of Ethanol Extract On Stems. Leaves. And Roots Of Citronella Grass ( Cymbopogon Nardus L.). BEST JOURNAL (Biology Education. Science. & Technology). 5(1). 141–147.
  • Takeuchi. H.. & Sakano. H. (2014). Neural Map Formation In The Mouse Olfactory System. Cellular And Molecular Life Sciences. 71(16). 3049–3057. Https://Doi.Org/10.1007/S00018-014-1597-0
  • Tibenda. J. J.. Yi. Q.. Wang. X.. & Zhao. Q. (2022). Review Of Phytomedicine. Phytochemistry. Ethnopharmacology. Toxicology. And Pharmacological Activities Of Cymbopogon Genus. Frontiers In Pharmacology. 13. Https://Doi.Org/10.3389/Fphar.2022.997918
  • Wells. D. L. (2001). The Effectiveness Of A Citronella Spray Collar In Reducing Certain Forms Of Barking In Dogs. Applied Animal Behaviour Science. 73(4). 299–309. Https://Doi.Org/10.1016/S0168-1591(01)00146-0
  • Yulianita. Y.. Effendi. E. M.. & Firdayani. E. M. (2019). Sedative Effect Of Citronella (Cymbopogon Nardus (L.) Rendle) Towards Male Mice (Mus Musculus). Indonesian Journal Of Pharmaceutical Science A

Downloads

Download data is not yet available.

Downloads

Published

2024-12-31

How to Cite

Surachmat, H. L., Arianto, E. F., & Khairani, S. (2024). Sedative Effect of Lemongrass (Cymbopogon nardus) Aromatherapy through Inhalation Route on Mice (Mus musculus) Behavior. Veterinary Biomedical and Clinical Journal, 6(2), 70–78. https://doi.org/10.21776/ub.VetBioClinJ.2024.006.02.3

Issue

Vol. 6 No. 2 (2024):

Section

Articles

License

Copyright (c) 2024 Hilmy Luqman Surachmat, Eko Fuji Arianto, Shafia Khairani

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.