THE STUDY OF ORGANOPHOSPHATE (DIAZINON) TOXICITY TOWARD LIVER HISTOPATHOLOGY AND MALONDIALDEHYDE (MDA) SERUM LEVELS ON RATS (Rattus norvegicus)
Keywords:
diazinon, malondialdehyde (MDA), liver histopathologyAbstract
Diazinon is an insecticide that has a higher toxicity than other insecticides. Normally, insecticides are detoxified by liver, but this process produced free radicals which causes cell damage. Free radicals also increase lipid peroxidation which directly increase malondialdehyde levels. This research was aimed to determine the influence of diazinon toxicity to the liver histopathology and levels of malondialdehyde (MDA) in the serum of rats (Rattus norvegicus). The rats were divided into 4 groups which consist of negative control group and three experimental groups which were given diazinon 20 mg/kgBW (P1), 40 mg/ kgBW (P2), and 60 mg/ kgBW (P3). The rats were given diazinon for 8 weeks orally. The parameters used in this research was liver histopathology with hematoxilin eosin stain and Malondialdehyde levels with Thiobarbituric test. Liver histopathology was analyzed in descriptive qualitative and the level of MDA was analyzed quantitatively using ANOVA and Tukey's exact test with α = 0.05. The result of this research showed the influence of organophosphate (diazinon) on liver histolopathology was shown by inflammatory cells infiltration in hepatic parenchyma, sinusoidal congestion, and cytoplasmic vacuolation of the hepatocytes. Statistical analysis proved that the diazinon was able to increase the level of MDA serum significantly (p<0,05) up to 71% in experimental group P1 (dose 20 mg/kgBW), which the increase level of MDA serum depend on dose of diazinon. This research concluded that diazinon is one of the organophosphate pesticide that toxic based on liver histopathology and MDA serum levels.
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References
Ameriana, M. 2006. Perilaku Petani Sayuran dalam Menggunakan Pestisida Kimia. Balai Penelitian Tanaman Sayuran. Bandung.
Amilia, E. 2016. Residu Pestisida pada Tanaman Holtikultura (Studi Kasus di Desa Cihanjuang Rahayu Kecamatan Parongpong Kabupaten Bandung Barat. Jurnal Agrikultura 27 (1): 23-29.
Anggraini, D. R. 2008. Gambaran Makroskopis dan Mikroskopis Hati dan Ginjal Mencit Akibat Pemberian Plumbum Asetat. Universitas Sumatera utara. Medan.
Aristantyo, A. 2013. Hubungan Aktivitas Asetilkolinesterase Darah dengan Kejadian Hipotensi Ortostatik pada Petani. Universitas Diponegoro. Semarang.
Azis, T. 2011. Analisis Residu Pestisida Diazinon dalam Tanaman Kubis (Brassica Olarecea) Menggunakan Biosensor Elektrokimia Secara Voltametri Siklik. Jurnal Prog. Kim. Si 1 (1): 32-40.
Baconi, D. L., M. Barca, G. Manda, A. Ciobanu, and C. Balalau. 2013. Investigation of the Toxicity of Some Organophosphorus Pesticides in a Repeated Dose Study in Rats. Rom J Morphol Embryol 2013, 54(2):349-356
Cesta, M. F. 2014. NTP Nonneoplastic Lesion Atlas. <https://ntp.niehs.nih.gov/nnl/hepatobiliary/liver/inflamm/liver-inflammation_508.pdf> [Diakses pada tanggal 21 Juni 2017 Pukul 08;04 WIB]
Cox, C. 2000. Diazinon: Toxicology. Journal of Pesticide Reform / Summer 2000 Vol. 20, No. 2.
Debski, B., B. F. Kania, and T. Kuryl. 2007. Transformations off Diazinon, an Organophosphate Compound in The Environment and Poisoning by This Compound. Journal Ekologia (Bratislava) Vol. 26, No. 1 p. 68-82.
Edem, VF., A. Kosoko, S.B. Akinyoola, O. Owoeye, S.K. Rahamon, and O.G. Arinola. 2012. Plasma Antioxidant enzymes, lipid Peroxidation and Hydrogen Peroxide in Wistar Rats Exposed to Dichlorvos Insecticide. Archives of Applied Science Research 4(4): 1778-1781
Elersek, T., and F. Metka. 2011. Organophosphorus Pesticides- Mechanisms of Their Toxicity. National Institute of Biology. Slovenia.
El-Shenawy, N. S., R. A. Al-Eisa, F. El-Salmy, and O. Salah. 2009. Prophylactic Effect of Vitamin E against Hepatotoxicity, Nephrotoxicity, Haematological induces and Histopathology induced by Diazinon Insetde in Mice. Curr Zool., 55 (3): 219-226.
Ginting, M. 2014. Gambaran Pengetahuan Petani Jeruk tentang Keracunan Akibat Penggunaan Pestisida di Kecamatan Tigapanah Kabupaten Karo [SKRIPSI]. Fakultas Keperawatan. Universitas Sumatera Utara
Hasan, S. A., I. M. El-Shawaf, A. El-Ghazaly, and S. M. El-Azab. 2007. Ozone Administration Ameliorates Different Chemically induced Hepatorenal Chronic Toxicity in Rats: a Histopatological Study. Mansoura Journal Forensic Med. Clin. Toxicol. Vol XV, (2): 57-67
Hreljac, I. 2009. Organophosphorus Pesticides Enhance The Genotoxicity of Benzo(a)pyrene by Modulating its Metabolism. Mut Res 671: 84-92
Jackson, S., C. Groves. Taxonomy of Australian Mammals. CSIRO Publishing. Clayton South.
Kusriani, 2012. Uji Pengaruh Sublethal Pestisida Diazionon 60 EC terhadap Rasio Konversi Pakan (FCR) dan Pertumbuhan Ikan Mas (Cyprinus carpio L.). Jurnal Penelitian Perikanan 1 (1): 36-42.
Latifa, K. I. 2015. Profil Kadar MDA (Malondialdehyde) pada Tikus yang Diberikan Ekstrak Herba Thymi ( Thymus vulgaris [L.]) [SKRIPSI]. Fakultas Farmasi. Universitas Muhammadiyah Surakarta
Luthfanto, H. 2014. Hubungan Antara Aktivitas Asetilkolinesterase Darah dan Arus Puncak Ekspirasi Petani Kentang dengan Paparan Kronik Pestisida Organofosfat. Universitas Diponegoro. Semarang
Masyitha, D. 2006. Struktur Mikroskopik Tulang Mandibula pada Tikus Ovariektomi dan Pemberian Pakan Rasio Fosfat/ Kalsium Tinggi. Universitas Syiah Kuala. Banda Aceh.
Petcoff, G.M., A.O Diaz, A.H. Escalante, and A.L. Goldemberg. 2006. Histology of The Liver of Oligosarcus jenysii (Ostariophysi, Characidae) from Los Padres Lake, Argentina. Iheringia, Ser. Zool., Porto Alegre, 96(2):205-208,30.
Prahanarendra, G. 2015. Studi Awal Histoteknik: Gambaran Histologi Organ Ginjal, Hepar, dan Pankreas Tikus Sprague dawley dengan Pewarnaan HE dengan Fiksasi 3 Minggu. UIN Syarif Hidayatullah. Jakarta.
Roberts, J. R. 2013. Recognition and Management of Pesticide Poisoning. U.S. Environmental Protection Agency. Washington, DC.
Ryczek, A.T., B.T. Deptula, W. Deptula. 2011. Immunity of The Liver. Central European Journal of Immunology 2011: 36 (3).
Sarhan, O. M. M., Z. Y. Al Sahhaf. 2011. Histological and Biochemical Effects of Diazinon on Liver and Kidney of Rabbits. Life Science Journal, 2011; 8 (4).
Singh, Z. 2014. Use of Malondialdehyde as a Biomarker for Assessing Oxidative Stress in Diferent Disease Pathologies: a Review. Iranian Journal Public Health, Vol. 43 Suppl. No.3 pp: 7-16.
Suhita, N. L. P. R., I W. Sudira, I. B. O. Winaya. 2013. Histopatologi Ginjal Tikus Putih Akibat Pemberian Ekstrak Pegagan (Centella asiatica) Peroral. Buletin Veteriner Udayana Vol. 5 No. 2
Uchendu, C., S.F. Ambali, and J.O. Ayo. 2012. The Organophosphate, Chlorpyrifos, Oxidative Stress and The Role of Some Antioxidants: A Review. African Journal of Agricultural Research Vol 7 (18). Pp 2720-2728.
Valko, M., C. J. Rhodes, J. Moncol, M. Izakovic, and M. Mazur. 2007. Free Radical, Metal and Antioxidants in Normal Physiological Function and Human Diseases. Inter J Biochem Cell Biol. 2007;39 44-84
Widiartini, W. 2013. Pengembangan Usaha Produksi Tikus Putih (Rattus norvegicus) Tersertifikasi dalam Upaya Memenuhi Kebutuhan Hewan Laboratorium. Universitas Diponegoro. Semarang.
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