The Ointment of Peel Dragon Fruit (Hylicereus costaricensis) Extract Decreased the Interleukin-2 (IL-2) Expression and the Number of Mononuclear Inflammation Cells toward Open Wound in Skin of Rats Wistar
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https://doi.org/10.21776/ub.VetBioClinJ.2019.001.02.4Keywords:
open wound, extract of dragon fruit peel, IL-2, mononuclear inflammatory cellsAbstract
Open wounds healing is need more time because of the long inflammatory phase. Dragon fruit skin contains flavonoids and vitamin C which function as anti-inflammatory and cell regeneration. The purpose of this study was to determine the therapeutic effects of dragon fruit skin extract ointment (Hylicereus costaricensis) on the area of IL-2 expression and the number of mononuclear inflammatory cells in skin tissue in open wounds of rats (Rattus norvegicus) for 6 days of treatment. As many as 20 male rats, ± 180 grams, Wistar were divided into 5 groups namely negative control, positive control, ointment of dragon fruit skin extract with concentration of 5% (P1), concentration of 10% (P2) and concentration of 15% (P3). Wound formed as dorsal skin biopsy of dorsal 1x1 cm. Ointment was given topically 2x/day for 6 days. Rats were euthanized on day 7th and the skin tissues of the wound area were collected. The number of mononuclear inflammatory cells was counted in skin slides of HE staining and Expression Area expression of IL-2 was measured by ImmunoRatio® in immunohistochemical slides. Data were analyzed statistically using One Way ANOVA and Tukey Post Hoc Test. The results showed that dragon fruit skin extract ointment was able to reduce significantly (p<0.05) for area expression of IL-2 and the number of mononuclear inflammatory cells and the best concentration of ointment for treatment was 15%. This is due to the effect of flavonoids as anti-inflammatory and vitamin C as a trigger for cell regeneration. The conclusion of the study was that ointment of dragon fruit peel able to become a candidate for therapeutic medicine for open wounds.
References
- Asep E. Sukmayadi, Sri A. Sumiwi, Melisa I. Barliana, Anisa D. Aryanti, 2014. Aktivitas Imunomodulator Ekstrak Etanol Daun Tempuyung (Sonchus arvensis Linn). Fakultas Farmasi, Universitas Padjadjaran, Sumedang, Jawa Barat, Indonesia
- Bratawidjaja, K., dan I. Rengganis. 2014. Imunologi Dasar. Badan Penerbit Fakultas Kedokteran. Universitas Indonesia. Jakarta. 319.
- Broughton, I., J. Janis, and E. Attiger. 2006. Wound healing : an overview. Plastic Reconstruction Surgery 117 (supplement) : 1eS-32eS. Dalas, texas
- Cahyono, B. 2009, Buku Terlengkap Sukses Bertanam Buah Naga, Pustaka Mina, Jakarta.
- Elias J.M., M. Margiotta, D. Gaborc. 1989. Sensitivity and detection efficiency of the peroxidase antiperoxidase (PAP), avidin-biotin peroxidase complex (ABC), and peroxidase-labeled avidin-biotin (LAB) methods. Department of Pathology, Health Sciences Center, Stony Brook, New York.American Journal of Clinical Pathology[1989, 92(1):62-67
- Jaafar. A., R. Nazri, dan W. Khairuddin. 2009. Proximate Analysis of Dragon Fruit (Hylecereus polyhizus), American Journal of Applied Sciences, 6 : 1341-1346.
- Kurniawati, A. 2005. Uji Aktivitas Anti Inflamasi Ekstrak Metanol raptophyllum griff pada Tikus Putih. Majalah Kedokteran Gigi Edisi Khusus Temu Ilmiah Nasional IV, 11-13 Agustus 2005: 167-170.
- Li, J., J. Chen, and R. Kirsner. 2007. Pathophysiology of acute wound healing. Clinics in Dermatology. Vol: 25. pp. 9-18.
- Naibaho, O., V. Paulina, dan W. Weny. 2013. Pengaruh Basis Salep terhadap Formulasi Sediaan Salep Ekstrak Daun Kemangi (Omicum sanctum L.) pada Kulit Punggung Kelinci yang Dibuat Infeksi Staphylococcus aureus. Jurnal Ilmiah Farmasi UNSTRAT Vol. 2 No. 02.
- Nijveldt R, van Nood E, van Hoorn DEC, Boelens PG, van Norren K, van Leeuwen AM. 2001 Dalam Rahmawati, G., F.N. Rachmawati., H. Winarsi., 2014.Aktivitas Superoksida Dismutase Tikus Diabetes Yang Diberi Ekstrak Batang Kapulaga Dan Glibenklamid. Fakultas Biologi. Universitas Jenderal Soedirman. Purwokerto
- Potter, P. 2007. Rats and Mice: Introduction and use In Research. Health Sciences Center for Educational Resources university of Washington.
- Setiabudi, A. 2005. Perbandingan Ekspresi Sel T CD4 di Jaringan Sekitar luka Dengan Tanpa infiltrasi Levobupivaksin Pada Nyeri Pasca Incisi. Program Magister Ilmu Biomedik dan PPDS J Universitas Diponegoro Semarang. [Tesis]
- Smith dan Mangkoewidjojo. (1998) dalam Nasution Nurhayati. 2015. Uji Aktifitas Ekstrak Etanol Umbi Talas Jepang (Colocasia esculenta(L) Schott var. Antiquorum) Terhadap Penyembuhan Luka Terbuka Pada Tikus Putih (Rattus novergicus) Jantan Galur Sprague Dawley. Farmasi. UIN Syarif Hidayatullah. Jakarta.
- Yenti, R., R. Afrianti., L. Afrianti. 2011 dalam Nasution Nurhayati. 2015. Uji Aktifitas Ekstrak Etanol Umbi Talas Jepang (Colocasia esculenta(L) Schott var. Antiquorum) Terhadap Penyembuhan Luka Terbuka Pada Tikus Putih (Rattus novergicus) Jantan Galur Sprague Dawley. Farmasi. UIN Syarif Hidayatullah. Jakarta.
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Published
2019-08-06
How to Cite
Permata, F. S., & Febrianto, A. (2019). The Ointment of Peel Dragon Fruit (Hylicereus costaricensis) Extract Decreased the Interleukin-2 (IL-2) Expression and the Number of Mononuclear Inflammation Cells toward Open Wound in Skin of Rats Wistar. Veterinary Biomedical and Clinical Journal, 1(2), 72–82. https://doi.org/10.21776/ub.VetBioClinJ.2019.001.02.4
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Copyright (c) 2019 Fajar Shodiq Permata, Ahmad Febrianto
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