The Identification of Hysterothlacium on Sixbar Groupers (Epinephelus sexfasciatus) in The Territorial Waters of East Java Using Sequencing Method
Identifikasi Hysterothylacium sp. pada Ikan Kerapu Belang (Epinephelus sexfasciatus) di Perairan Jawa Timur Menggunakan Metode Sekuensing
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DOI:
https://doi.org/10.21776/ub.VetBioClinJ.2023.005.01.2Keywords:
Ephinephelus sexfasciatus, Hysterothylacium amoyense, molecular identification, PCR, squencingAbstract
This study aimed to identify the parasitic nematode Hysterothylacium sp. using the sequencing method. A sampling of Hysterothylacium sp. was taken from 60 striped grouper fish (Ephinephelus sexfasciatus) and has characteristics such as brown color, booring teeth on the anterior, a simple digestive system including the esophagus, stomach (ventriculus), small intestine and does not have mucron on the posterior. Specific primers for the COX-2 mtDNA gene (210F and 211R) were used for DNA amplification (Polymerase Chain Reaction) and DNA sequencing with a length of 629bp. The positive result of the PCR method (sample S) was sequenced. Then, the results of the sequencing method were compared with the reference DNA sequences KY065298.1, MF120253.1, MF120248.1, and KU886687.1. Furthermore, Homology and kinship tests were carried out using GenBank (NCBI). Results showed a 97% similarity of the sample with Hysterothylacium amoyense cytochrome oxidase subunit II (COX-2) gene MF120253.1.
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Published
2023-07-19
How to Cite
Kismawardani, P., Koesdarto , S., Santoso , K. P., Lastuti , N. D. R., Sabdoningrum , E. K., & Kusnoto. (2023). The Identification of Hysterothlacium on Sixbar Groupers (Epinephelus sexfasciatus) in The Territorial Waters of East Java Using Sequencing Method: Identifikasi Hysterothylacium sp. pada Ikan Kerapu Belang (Epinephelus sexfasciatus) di Perairan Jawa Timur Menggunakan Metode Sekuensing. Veterinary Biomedical and Clinical Journal, 5(1), 11–18. https://doi.org/10.21776/ub.VetBioClinJ.2023.005.01.2
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Copyright (c) 2023 Putri Kismawardani, Setiawan Koesdarto , Kuncoro Puguh Santoso , Nunuk Dyah Retno Lastuti , Emy Koestanti Sabdoningrum , Kusnoto
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