The Identification of Hysterothlacium on Sixbar Groupers (Epinephelus sexfasciatus) in The Territorial Waters of East Java Using Sequencing Method

Identifikasi Hysterothylacium sp. pada Ikan Kerapu Belang (Epinephelus sexfasciatus) di Perairan Jawa Timur Menggunakan Metode Sekuensing

Authors

Putri Kismawardani
Mahasiswa Fakultas Kedokteran Hewan, Universitas Airlangga Surabaya
Setiawan Koesdarto
Departemen Parasitologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga Surabaya
Kuncoro Puguh Santoso
Departemen Kedokteran Hewan Dasar, Fakultas Kedokteran Hewan, Universitas Airlangga Surabaya
Nunuk Dyah Retno Lastuti
Departemen Parasitologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga Surabaya
Emy Koestanti Sabdoningrum
Departemen Peternakan, Fakultas Kedokteran Hewan, Universitas Airlangga Surabaya
Kusnoto
Departemen Parasitologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga Surabaya

Page: 11-18, Abstract views: 542, PDF downloads: 548

DOI:

https://doi.org/10.21776/ub.VetBioClinJ.2023.005.01.2

Keywords:

Ephinephelus sexfasciatus, Hysterothylacium amoyense, molecular identification, PCR, squencing

Abstract

This study aimed to identify the parasitic nematode Hysterothylacium sp. using the sequencing method. A sampling of Hysterothylacium sp. was taken from 60 striped grouper fish (Ephinephelus sexfasciatus) and has characteristics such as brown color, booring teeth on the anterior, a simple digestive system including the esophagus, stomach (ventriculus), small intestine and does not have mucron on the posterior. Specific primers for the COX-2 mtDNA gene (210F and 211R) were used for DNA amplification (Polymerase Chain Reaction) and DNA sequencing with a length of 629bp. The positive result of the PCR method (sample S) was sequenced. Then, the results of the sequencing method were compared with the reference DNA sequences KY065298.1, MF120253.1, MF120248.1, and KU886687.1. Furthermore, Homology and kinship tests were carried out using GenBank (NCBI). Results showed a 97% similarity of the sample with Hysterothylacium amoyense cytochrome oxidase subunit II (COX-2) gene MF120253.1.


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Published

2023-07-19

How to Cite

Kismawardani, P., Koesdarto , S., Santoso , K. P., Lastuti , N. D. R., Sabdoningrum , E. K., & Kusnoto. (2023). The Identification of Hysterothlacium on Sixbar Groupers (Epinephelus sexfasciatus) in The Territorial Waters of East Java Using Sequencing Method: Identifikasi Hysterothylacium sp. pada Ikan Kerapu Belang (Epinephelus sexfasciatus) di Perairan Jawa Timur Menggunakan Metode Sekuensing. Veterinary Biomedical and Clinical Journal, 5(1), 11–18. https://doi.org/10.21776/ub.VetBioClinJ.2023.005.01.2

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